Marker
Bacteria & Archaea

Paste a full-length rRNA sequence (50-5000 bp). Predictions are returned across all eight taxonomic ranks with confidence scores. Try a Bacterial example.

Classification

Upload a FASTA or FASTQ file (gzipped supported). Each sequence is classified individually and a community-level abundance summary is provided. Maximum 150 MB.

Drop your file here or click to browse
.fasta · .fa · .fastq · .fq · .gz
Limit sample size Off by default — all reads in the file are processed. Turn on to cap how many reads to process (e.g. a faster preview).
Skip adapter trimming Adapter trimming now runs automatically when ONT adapter signatures are detected in more than 5% of sampled reads. Enable this only if your reads are already adapter-trimmed.
Minimum quality (FASTQ only) Phred score threshold. 0 disables filtering. 7 is the permissive Nanopore default.
0
Custom length filter Off by default — reads are filtered to the marker's validated window (16S 1,200–2,000 bp, ITS 300–1,000 bp). Turn on to set your own range.
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Processing pipeline
Taxonomic abundance
AI Generated summary

Upload up to 10 FASTA/FASTQ files (1 GB total) and add metadata for downstream comparison. Metadata can be entered in the table or uploaded as a CSV/XLSX with a filename column.

Drop multiple files here or click to browse
Skip adapter trimming Adapter trimming runs automatically for each sample when ONT adapter signatures are detected in more than 5% of sampled reads. Enable this only if your reads are already adapter-trimmed.
Limit sample size Off by default — all reads in every sample are processed. Turn on to cap how many reads per sample.
Custom length filter Off by default — every sample is filtered to the marker's validated window (16S 1,200–2,000 bp, ITS 300–1,000 bp). Turn on to set your own range.
Processing samples
Multi-sample analysis